After searching in my computer, I found that booting.ini file was missing in my system.
Solution:
Go to run & type “msconfig”, and then go to Boot.ini tab.

[For me, there was nothing in Booting Script field & Timeout field.]
BOOT.ini:
[boot loader]
timeout=30
default=multi(0)disk(0)rdisk(0)partition(1)\WINDOWS
[operating systems]
multi(0)disk(0)rdisk(0)partition(1)\WINDOWS="Microsoft Windows XP Professional" /fastdetect
Note: This default Boot.ini file from a Windows XP Professional computer (verified at http://support.microsoft.com/kb/289022 ).
Copy these lines and paste it to Booting Script field. If this information is already present then don’t change it. Now click on “Check all Boot Paths”, you will get a message that everything is good and Timeout field will be field by “30” sec.
Now restart your system, you will not get that error message.